Cell signaling tech - Solutions for Neurodegeneration Drug Discovery. Download “Solutions for Advancing Neurodenerative Therapeutics Discovery” and discover more! An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for …

 
Cell signaling techCell signaling tech - Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed …DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career …NOTE: Most antibodies from Cell Signaling Technology work optimally between 1 and 2 ug per IP sample. In the case where there are multiple samples with varying concentrations, …CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and …Polyclonal Antibody for studying Flag-Tag. Cited in 774 publications. Validated for WB, IP, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #2368) is …Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7). Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates ...Since 2000, Cell Signaling Technology (CST) has offered a rigorously tested and well-cited polyclona... Charles Farnsworth, PhD Aug 9, 2023. A Quick Guide to Neuronal and Glial Cell Type Markers. The central nervous system (CNS) is composed of highly specialized cells, each of which play a key r...Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb.Background. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the ... Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected]. Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ... p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Western blot analysis was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Confocal immunofluorescent analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb ... Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes . Typically, the signaling process involves three components: the signal, the receptor, and the effector. [citation needed] In biology, signals are mostly chemical in nature, but can also be physical cues such as pressure, voltage, temperature, or light.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ...Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Dana Seehale joined Cell Signaling Technology in February 2017 as Director, Lab Informatics, responsible for multiple technologies supporting Product Development and Production, including PDP/PLM, Core LIMS, and Jira. Her responsibilities were later expanded to include the PIM/STEP system and the Oracle EBS/EPortal platforms. Dana is now ...Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells, transfected with a construct expressing HA-tagged Stat3 using HA-Tag (6E2) Mouse mAb #2367. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).Pathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ... The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). Our Approach/Process. As a privately held company founded and led by active research scientists, we understand your needs as a researcher. Like you, we only want to use products that are specific and deliver reproducible results. We validate every one of our thousands of antibodies and research products in-house -- and against multiple ... SignalStar Multiplex IHC from CST is a revolutionary new tool for spatial biology research, providing fully flexible and highly-validated antibody panels. SignalStar technology amplifies multiple biomarkers simultaneously in FFPE tissue with high sensitivity and specificity. Accurate, reliable data can be generated on up to 8 targets in just 2 ...As we continue to create and provide the highest quality of products and services, be part of the team that empowers scientists and clinicians with our best-in-class tools and excellent customer experiences. Engage and share your expertise with our current customers while helping drive results by converting potential clients into profitable ones.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Lab Expectations. The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information. 抗体不应成为您实验中的变量之一。了解客户为何在抗体特异性和敏感性方面给予 cst 最高评价。 p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ... IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Product Description. The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an ...H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3).Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ... Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on …The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA. Western blot analysis of extracts from COS-7 cells, untreated or hydroxyurea-treated (G1/S), using Rb (4H1) Mouse mAb. Immunohistochemical analysis of paraffin-embedded human breast ...Cell biology research seeks to understand all aspects of cell growth, survival, and death in the contexts of both health and disease. Understanding the complex and dynamic cellular landscape can provide insights into healthy and disease states. Cell Signaling Technology (CST) provides a diverse and comprehensive catalog of rigorously tested and ... Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase ... Cell Signaling Technology poster included with each book. The two-sided poster details important signaling pathways. Chapter 12, How Cells Make Decisions , offers a unique visual and interrogative approach that explores four key signaling pathways from teh organismal to molecular levels. IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) Antibody #9661. The virtual lane view (left) shows two target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the ... Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9). Refine Results. In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas. Cell Signaling presents the principles and components that underlie all known signaling processes. It provides undergraduate and graduate students the conceptual tools needed …In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ... Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins, such as occludin and claudin, to the actin cytoskeleton (reviewed in 2). ZO-1 and ZO-2 are required for …Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Science & Development. As a company of scientists for scientists, we are passionate about the discovery and development of innovative therapies for human disease. Help advance … Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. Product Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated BiP (C50B12) Rabbit mAb #3177.The LC3B (D11) XP ® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. Show More. Simple Western™ analysis of lysates (1mg/ml) from HeLa cells treated with Chloroquine (50uM, O/N) using LC3B (D11) XP ® Rabbit mAb #3868. Incubate membrane with Anti-mouse IgG, HRP-linked Antibody ( #7076 at 1:2000) and Anti-biotin, HRP-linked Antibody ( #7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Proceed with detection (Section D). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and …The innate immune system mainly consists of phagocytic cells, such as neutrophils, macrophages, natural killer cells, basophils, and others that target dangerous invading organisms. The cells of the adaptive immune system are T cells and B cells. Natural killer (NK) T cells have features of both innate and adaptive immune cells. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Refine Results. In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas. Partnerships & Licensing. As a trusted partner, CST is focused on building symbiotic relationships to maximize the value of products, technology, and intellectual property; whether ours or our partner’s. True innovation is collaborative. We have broad interest in technology licensing, scientific and technical collaborations, and commercial ... Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) Antibody #9661. The virtual lane view (left) shows two target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. 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Species Cross-Reactivity Key: Polyclonal Antibody for studying DRP1 (Ser616) phosphate. Cited in 343 publications. Validated for WB, IP, IF, F. Highly specific and rigorously validated in-house, Phospho-DRP1 (Ser616) Antibody (CST #3455) is ready to ship.. City jersey city nj

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Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed …Polyclonal Antibody for studying Flag-Tag. Cited in 774 publications. Validated for WB, IP, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #2368) is …The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652.Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. This is true whether the cell is a single-celled organism or part of a larger, more complex multicellular organism. Cells communicate with their environment through a process called signaling. Cell signaling is how the cell collects information and then responds with an action at the correct time. Signaling is the initial event associated with ...H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3).The western blot technique is a powerful tool to elucidate the complex signaling events that underlie biological processes and disease. This paper highlights critical steps in the …Solutions for Neurodegeneration Drug Discovery. Download “Solutions for Advancing Neurodenerative Therapeutics Discovery” and discover more! An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for …Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Elucidate the mechanisms that drive neurodegenerative disease - including the molecular and cellular events related to the structure and function of cell types found in the brain including neurons, microglia, oligodendrocytes, and astrocytes - with validated antibodies from Cell Signaling Technology (CST) for your research. Show More.Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ...Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic … Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ...Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb.Polyclonal Antibody for studying H3 (Ser10) phosphate. Cited in 1110 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) Antibody (CST #9701) is ready to ship. Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 and TCRβ . While you know that each protein is found at the plasma membrane, you need to check whether the antibodies recognize extracellular or intracellular portions of each. Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic …Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic …Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …The absence of signal in the HCT 116 knockout cells confirms the specificity of the antibody for LC3B. Western blot analysis of extracts from HeLa, C2C12, and KNRK cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +) #14774 using LC3B (E5Q2K) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Incubate membrane with Anti-mouse IgG, HRP-linked Antibody ( #7076 at 1:2000) and Anti-biotin, HRP-linked Antibody ( #7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Proceed with detection (Section D). Hr -Horse. -Guinea Pig. -Rabbit. -All Species Expected. Monoclonal Antibody for studying NeuN. Cited in 47 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST … For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... Specificity / Sensitivity. Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence. ELISA: An Introduction. Enzyme-linked immunosorbent assay (ELISA) allows for the quantification of a molecule of interest using antibodies and tags (such as the enzyme horseradish peroxidase [HRP]), either directly or indirectly coupled to the antibody for detection. ELISA can be used with a variety of sample types, such as cell lysate, tissue ... The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ... Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Show More. Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add.Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase ...CiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an …Immunoprecipitation of NF-kB p65 from CHO cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is NF-κB p65 (D14E12) XP ® Rabbit mAb, #8242. Western blot was performed using NF-κB p65 (L8F6) Mouse mAb, #6956. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Product Description. This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a G4S linker within the scFv of the extracellular domain. Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ...Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Product Description. Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase (HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST ...Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic ...Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein.Product Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated BiP (C50B12) Rabbit mAb #3177.At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.. Giorgio a tsoukalos, Greater rochester airport, Eyeglasses zenni, Cancercare, Mercedes fredericksburg, Ymca suncoast, Milanos pizza near me, Town 9 tap, Gen x clothing store.